Your lab uses the annelid worm Lumbriculus variegatus as a model organism to study regeneration. Your studies would be helped by knowing which genes are present in this organism, but its genome has not been sequenced. A recent technique for gene determination is RNA-Seq, in which RNAs are directly isolated and sequenced. What is a disadvantage of using RNA-Seq for gene identification?

a. RNA-Seq may miss genes transcribed in a very tissue- and temporal-limited fashion.
b. RNA-Seq relies on genes being homologous across species, but not all genes have homologs.
c. RNA-Seq works only in mammalian cells and therefore cannot be used with model organisms such as Lumbriculus or Drosophila.
d. RNA-Seq may selectively miss noncoding RNAs as compared to mRNAs.

Answers

Answer 1

Answer:

a. RNA-Seq may miss genes transcribed in a very tissue- and temporal-limited fashion.

Explanation:

RNA-Seq is a methodology used in molecular biology to identify RNA transcripts by using deep-sequencing techniques. RNA-Seq has shown that the genome is often transcribed in the form of non-coding RNA sequences such as, for example, long non-coding RNAs (lncRNAs), microRNAs (miRNAs), etc., thereby redefining our vision about transcription in eukaryotic organisms. This methodology is based on the conversion of RNAs to cloned cDNA (complementary DNA) fragments that are further sequenced to be mapped against the reference genome. In consequence, tissue-specific-transcripts are not detected by RNA-Seq in cells in which these sequences are not transcribed.


Related Questions

The most important prevention measure is controlling
temperature.
and
A
flavor
B
color
с
texture
D
time

Answers

Answer:

d.time

Explanation:

How does heat gases cause increase in pressure

Answers

Answer:

When a gas is trapped inside a container which has a fixed size (its volume cannot change) and the gas is heated, the particles will gain kinetic energy which will make them move faster. This causes the force on the walls of the container to increase and so the pressure increases.

Explanation:

Hope this helps

WHATS THE DEFFINITION OF HELLO??????????? ANSER FAST >:C

Answers

Answer:

It mean are you there

Explanation:

is this a real question

Hello, it's me

I was wondering if after all these years you'd like to meet

To go over everything

They say that time's supposed to heal ya

But I ain't done much healing

Hello, can you hear me?

I'm in California dreaming about who we used to be

When we were younger and free

I've forgotten how it felt before the world fell at our feet

There's such a difference between us

And a million miles

Hello from the other side

I must've called a thousand times

To tell you I'm sorry

For everything that I've done

But when I call you never

Seem to be home

Hello from the outside

At least I can say that I've tried

To tell you I'm sorry

For breaking your heart

But it don't matter, it clearly

Doesn't tear you apart anymore

Which of these involves estimating the size of a population by counting only the members of the population in a particular area and then using this to estimate the size of the entire population over its
entire inhabited area?

sampling

population index

counting the entire population

mark-and-recapture​

Answers

Answer:

counting the entire population

The term that involves estimating the size of a population by counting only the members of the population in a particular area is counting the entire population. The correct option is C.

What is a population?

The population is a term that is used for the number of people or particular species present in a specific area. The population of humans is calculated in specific areas.

The population growth rate is estimated by calculating the population of a particular place. This can be calculated by using the estimated size of the entire population over its entire inhabited area.

The death rate is minus from the population to get the growth rate of the population. The death rate is the number of people who died due to natural causes.

Thus, the correct option is C. counting the entire population.

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In which horizon is humus most concentrated?

A. A HORIZON
B. B HORIZON
C. C HORIZON
D. O HORIZON

Answers

Answer:

B. B HORIZON

Explanation:

Answer:

option A

Explanation:

Explain how each of Newton's Laws are demonstrated when a person plays golf. You may bullet your explanations as 1st, 2nd and 3rd Law.

Answers

It is why the people is the board in the house im in a house board

Imagine you are a carbon atom. Describe your journey through the carbon cycle be sure to include and explain the key processes in the cycle

(include the following in your answer)
 Describe how the carbon atoms in CO2 absorbed via plant photosynthesis provide the carbon atoms for all of the new carbon compounds a plant produces and uses to build its cells and tissues and to burn for energy.
 Explain how carbon compounds are transformed in five chemical reactions that are critical to the carbon cycle: photosynthesis, cell respiration, decomposition, biosynthesis and combustion.
 Explain how carbon moves from one Earth System sphere (neighborhood) to another
 Explain how carbon changes as it moves from one part of the carbon cycle to another
 Describe where carbon is stored

Answers

Answer:

The carbon cycle describes the process in which carbon atoms continually travel from the atmosphere to the Earth and then back into the atmosphere. Since our planet and its atmosphere form a closed environment, the amount of carbon in this system does not change.

1. Photosynthesis

Photosynthesis Process

Plants pull in carbon dioxide out of the air through photosynthesis. Even though carbon dioxide makes up less than 1% of the atmosphere, it plays a major role for living things.

With CO2 and H2O in the atmosphere, photosynthesis produces sugars like glucose. This is the plant material that plants synthesize on their own.

If you have the right conditions, this process can repeat for centuries. Not only does photosynthesis pulls carbon dioxide out of the atmosphere, but it’s fuels all living things as a source of energy.

2. Decomposition

Nutrient Cycle

By mostly using sunlight, water and carbon dioxide, plants can grow. In turn, animals consume food for energy using O2 and giving off CO2. Alternatively, they die, decay and decompose repeating for millions of years.

Decomposition is the process of breaking down plants. Over vast periods of time, layers of sediment build on each other. Because of the pressure and heat from within the Earth’s crust, this generates fossil fuels. Much of this happened during the Carboniferous Era.

For example, coal, oil and natural gas (methane) are some of the common fossil fuels. Over the long-term, the decomposition of dead matter generates these fossil fuel products.

Anaerobic decomposition involves bacteria breaking down organic matter such as glucose into CO2 and methane (CH4). The nutrient cycle recycles inorganic and organic material in soil through the process of decomposition. Then, it goes back again through the same process again.

3. Respiration

Cellular Respiration

You and I are both made of carbon. We consume plants. But we also breathe in the air, which has carbon in the form of carbon dioxide.

Animals rely on plants for food, energy and oxygen. Our cells require oxygen to break down the food we consume through cellular respiration.

Once consumed, carbon dioxide is released into the atmosphere because of cell respiration. In turn, this CO2 produced from respiring cells can be used in photosynthesis again.

In other words, the plants use solar energy to break apart that same carbon dioxide in the air. Through photosynthesis, it uses that same carbon for plant material in turn releasing oxygen again.

4. Combustion

Gas Combustion

Our cars use the energy released by burning fossil fuels. And carbon is also a pollutant as carbon dioxide.

We extract fossil fuels, combustion involves burning them to release energy. But a by-product of combustion is that it releases carbon dioxide back into the atmosphere. And too much CO2 increases the greenhouse effect.

And as we deplete our oil reserves adding CO2 into the air daily, this affects the carbon cycle with an imbalance of oxygen and carbon. Carbon dioxide is one of the greenhouse gases contributing to climate change.

But there is a limit for how much fossil fuels we can extract. Over millions of years, phytoplankton resting on the ocean surface photosynthesizes and takes in CO2.

Using sunlight, it creates a molecule called glucose (C6H12O6) and sinks to bottom of the ocean. Humans discovered these fossil fuels beneath the ocean. We started to drill the ancient plankton, which over millions of years ago, became the oil we use today.

Explanation:

HOPE IT HELPS : )

The periodic table is arranged according to ________________ atomic number.

Answers

Answer:

In the periodic table the elements are arranged in columns and rows according to increasing atomic number atomic number,

Explanation:

Ascending or increasing order of atomic number

11. Which type of ecosystem is the most productive?
river
coral reef
tropical rainforest
deciduous forest
desert
swamp
farm field

Answers

Answer:

tropical rainforest i think

Explanation:

Answer:

Swamps are the most productive

Explanation:

Because it produces more bacteria and in terms of the NPP unit rate its listed as the number 1

ANSWER THESE SIX QUESTIONS PLEASE, I WILL GIVE BRAINLIEST!!!



1. Define "cell" - tell what it is.


2. which part contains genetic information in these cells?
• Eukaryotic cell:
• prokaryotic cell:

3. define eukaryotic:

4. define prokaryotic:

5. what is the difference between intercellular communication and intracellular communication?

6. what are plasmodesmata?​

Answers

Answer:

Cells are the basic building blocks of all living things. ... Cells also contain the body's hereditary material and can make copies of themselves. Cells have many parts, each with a different function. Some of these parts, called organelles, are specialized structures that perform certain tasks within the cell.

The main difference between eukaryotic and prokaryotic cells is that eukaryotic cells have a nucleus. The nucleus is where cells store their DNA, which is the genetic material. The nucleus is surrounded by a membrane. Prokaryotic cells do not have a nucleus.

Eukaryote, any cell or organism that possesses a clearly defined nucleus. The eukaryotic cell has a nuclear membrane that surrounds the nucleus, in which the well-defined chromosomes (bodies containing the hereditary material) are located.

a microscopic single-celled organism that has neither a distinct nucleus with a membrane nor other specialized organelles. Prokaryotes include the bacteria and cyanobacteria.

What is the difference between intracellular signaling and intercellular signaling? Intracellular signaling occurs within a cell, and intercellular signaling occurs between cells.

a narrow thread of cytoplasm that passes through the cell walls of adjacent plant cells and allows communication between them.

Why would an airplane flying at 10,000 meters above the ground have more gravitational potential energy than the same airplane flying at 1,000 meters above the ground?

Answers

Answer: because the

Explanation: A flying airplane has potential energy has it flies above the ground level. And since the airplane is flying motion is associated with it and thus possesses kinetic energy.

The plane at 10,000 m will have more gravitational potential energy because it is directly proportional to height.

What is gravitational potential energy?

The energy stored by an object as a result of its position is called potential energy.

When an object is at a height, it is able to store gravitational potential energy. This energy is because of the attractive force of gravity acting on the object as a result of its height.

Gravitational potential energy is mainly dependent on two factors. These are - the mass of the object held at a height and the height at which it is held i.e the vertical distance between the object and the earth.

Gravitational potential energy is equal to

P E = m × g × h

Where m is the mass of the object

            g is the acceleration due to gravity

            h is the height

Therefore, gravitational potential energy is directly proportional to the mass of the object and the height at which it is held.

Since in the above case, we are talking about the same airplane so mass will be common. But at a height of 10,000m gravitational potential energy will be considerably higher compared to at a height of 1000 m.

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1. Tendons and ligaments are similar because they both have: a limited blood supply and low metabolism an abundant blood supply and low metabolism a limited blood supply and high metabolism an abundant blood supply and high metabolism 2. What is the name of the outer layer of connective tissue on skeletal muscle

Answers

Answer:

1 a limited blood supply and low metabolism

2. Epimysium

Explanation:

1.

The similarity that exists between tendons and ligaments from the question is low blood supply and also low metabolism. Ligaments do connect one bone to another bone while tendons connect bones to muscles.

2. Epimysium is the outermost layer of connective tissue that surrounds a skeletal muscle it is a Connective tissue layer which surrounds an individual muscle.

Fill in the blanks with the correct word

1- the male reproductive cell is called______


2- the female reproductive is called_______


3- when the male and female reproductive cells join during fertilization a_____ forms

Answers

Answer:

1. sperm

2. ova

3. zygote

Explanation:

The answer for number one is sperm number two ova

How does slow movement aid sloths in acquiring food?

Answers

Answer:

Sloths are so slow moving that algae is able to grow symbiotically in their fur. This algae can be licked out of the sloth's fur to provide it with nourishment.

Explanation:

The slow movement aid sloth in acquiring food, as slow and limited movements need less food and nutrition. It also helps in digesting food and the metabolism also works slowly.

What are sloths?

Sloths are arboreal Neotropical mammals that are very slow-moving and are herbivores, they eat leaves and algae, and twigs. They live in evergreen forests and tropical rainforests.

They are so slow that algae grow above their furs, which helps them camouflage between green forests from predators. Because they are quite adept at concealment. They not only live nearly entirely in trees and only venture out if absolutely necessary, but they also harbor algae in their fur that help them blend in.

Therefore, due to their limited and slow movements, sloths require less food and nutrition when foraging for food. Additionally, it aids in food digestion and slows down metabolism.

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The organelle that is made up of DNA:

Answers

The organelle that is made up of dna is the Nucleus

What effect did removing predators have on prey?

What effect did removing predators have on prey?

Answers

Answer:

Removing a predator can throw a ecosystem of its flow

Explanation:

If you where to take a wolf from an biome and it got its energy from deer. The deer would overpopulate. With that happening  its environment cant support all of them. Sooner or later the will not have enough food and die off.

Answer:

Removing a predator can throw a ecosystem off

Explanation:

Which of these is NOT true about the outer core?

A. It has a higher temperature than the inner core.

B. It is mostly made up of iron and nickel.

C. It is mostly liquid.

D. Atoms within it form a powerful magnetic field.

Answers

Answer:

B isnt true

Explanation:

Answer: the answer is It has a higher temperature than the inner core. for k12

Explanation:

why would gel filtration chromatography be the best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies

Answers

Answer: The question is incomplete and the options are not given, from my searches I was able to get the completed part which are;

A. Antibodies can be used to detect the antigen on a western blot

B. Antigens can be centrifuged through a column and pellet at specific speeds C. Antigens can easily be purified by charge and size when coated with SDS D. Antigens will separate by size going through the column beads and their holes

E. Organelles containing the antigen will have a specific buoyancy and separate in a gradient.

The correct option is D.

Antigens will separate by size going through the column beads and their holes

Antibodies can be used to detect the antigen on a western blot

Explanation:

The best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies by gel filtration chromatography is Antigens will separate by size going through the column beads and their holes because Gel filtration chromatography is a size exclusion chromatography that work on the principle that molecules are separated based on their big sizers or larger sizes. The columns are designed to remove substances that are smaller in sizes. The larger size antigen elute first.

Water molecules attract one another via:​

Answers

Answer:

Hydrogen Bonds

The slight positive charges on the hydrogen atoms in a water molecule attract the slight negative charges on the oxygen atoms of other water molecules. This tiny force of attraction is called a hydrogen bond. This bond is very weak.

Explanation:

hope this helps you ✌️✌️

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The law of conservation of mass states that during a reactions atoms are
________.

destroyed

created

rearranged

Answers

Rearranged
This is because they cannot be created or destroyed in the conversation of mass
Rearranged! I hope this helps!

3) A student conducts an investigation involving the identification all species of plants and animals within an area of a forest. What level of ecological organization is the student studying? A) Population B) Community C) Biosphere D) Ecosystem​

Answers

Answer:

D. Ecosystem

Explanation:

Sana nakatulong!!

When communities form in previous uninhabited areas, this is called...?

Answers

Answer:

sucession

Explanation:

When does exponential growth of a population occur?
A. When the ecosystem has biodiversity
B. When a population crash is occurring
C. When needs exceed resources
D. When the population is below carrying capacity
SUBMIT

Answers

Answer:

It’s D

Explanation:just took the test on ape

g The Drosophila melanogaster genome contains approximately 24 million bases of exon sequence, 20 million bases of intron sequence, and 76 million bases of intergenic sequence. Calculate and compare the percentages of exon and intron sequences in Drosophila melanogaster genome to that of the human genome.

Answers

Answer:

When it is compared to the human genome, the Drosophila melanogaster genome contains a higher proportion of exons (20%) and a minor proportion of introns (16.6%).

Explanation:

The Drosophila melanogaster genome contains 24 million bases (Mb) encoded by exons + 20 Mb introns + 76 Mb intergenic sequences = 120 Mb >>

- Exon sequences= (24 x 100)/120: 20 %

- Intron sequences= (20 x 100)/120: 16.6 %

- Intergenic sequences= (76 x 100)/120: 63.3 %

On the other hand, the completion of the human genome sequencing showed that only 1.1% encodes proteins (i.e., they are exons), 24% are introns, and 75% are intergenic sequences. Moreover, it is also important to highlight that the D. melanogaster genome contains about 13,000 protein-coding genes, while the human genome contains nearly 22,000-30,000 protein-coding genes.

What is the event called when a population travels to another area to live and reproduce?
selection
mutation
evolution
nigration​

Answers

Answer:

Explanation:

migration

The Correct Answer:

     migration

3. (03.06 LC)
What is a wave?(2 points)
It describes the change in sound in which something goes from high to low.
It describes the flow of heat which flows from an area of high to low temperature.
It describes the movement of air in which something moves in a circular pattern.
It describes the pattern of motion in which something moves back and forth.

Answers

It describes the flow or heat which flows from an area of high to low temperature

The type of cell division where the daughter cells have exactly the same as the parent cells is called

budding
mitosis
symbiosis
meiosis

Answers

Answer:

Mitosis

Explanation:

Mitosis is a type of cell division where the cell replicates its DNA and splits into two identical daughter cells. This occurs in some types asexual reproduction or in cell proliferation.

Meiosis produces genetically diverse daughter cells with half the amount of DNA as the parent. It occurs as part of asexual reproduction.

During budding, the cell divides asymmetrically as part of asexual reproduction.

Symbiosis is when two organisms interact with one another, and is usually benefit to both of them. It is not a type of cell division

Answer:

Mitosis

Explanation:

A new organism develops from a small bud that forms on the parent. It grows into a copy of the parent through mitosis.

What is the result of meiosis ll

Answers

four diploid daughter cells are going to be formed

Explain the results of the experiment in which the diffusion bag contained 15% fructooligosaccharides.

Answers

The sugar concentration was lower in the beaker them in the bag, so water move from the beaker to the bag.

how many years would it take a radio signal from the exoplanet wolf 359 to reach earth? Wolf is 7.9 light years from earth.

Answers

Answer:

Radio waves move at the speed of light which is aproximately 300,000 km per second or 3.8*10^8 m/s

1 ly = 9.461*10^15 m

Then

7.9 light-years = 7.4*10^16 m

By, velocity = distance/ time

time = distance/time

t = 7.4*10^16/3.8*10^8

= 1.9473684*10^24 seconds

Converting seconds to years (1 year = 31536000 seconds)

1.9473684*10^24/ 31536000 years

=6.17096989 ×10^16 years

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