Why are the plasmid vector and EcoRI the appropriate choices for the creation of a recombinant plasmid containing the kanR gene?

Answers

Answer 1

Answer:

Production of these sticky ends is another feature of restriction enzymes that makes them suitable for recombinant DNA technology. ... Hence, if both vector DNA and donor DNA are cut with EcoRI, the sticky ends of the vector can bond to the sticky ends of a donor fragment when the two are mixed.


Related Questions

list 3 negative impacts that overpopulation can have on a population!!

Answers

Answer:

1. Elimination of resources

2. Un-sanitary conditions

3. Bad (mental/physical) Health

Select all the correct answers.
Betty learned about embryology in her science class. At home, her brother Nathan noticed the human embryo and the fish embryo in her science notebook. He remarked that the embryos looked the same. How can this be true?

Both embryos have pharyngeal arches.
The fish embryo has evidence of lungs.
The human embryo has scaly skin.
Both embryos have fins.
Both embryos have a notochord.

Answers

Answer:

They both have pharyngeal arches.

They both have notochords.

The both embryos of human and fish are the same because;They both have pharyngeal arches.They both have notochords.What is embryology?

The term embryology refers to the study of the branch of science that has to do with the study of development of the embryo of organisms. It provides powerful evidence for the theory  of evolution.

The both embryos of human and fish are the same because;

They both have pharyngeal arches.They both have notochords.

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Which of the following sentences uses spatial organization?

Answers

I believe B is the only logical answer.

The sentence which uses the spatial organization is option B . Describing the directions and characteristics of the lands nearby is an example of spatial organization.

What is spatial organization ?

When elements of an abiotic or biological group are placed in space in a non-random manner, spatial organization can be seen. After thousands of particles interact millions of times, abiotic patterns such as the ripple formations in sand dunes or the oscillating wave patterns are typical  illustration for spatial organization.

Disagreement between nations, reading habit, etc. does not describe anything related to space and surroundings.

The description of a landscape and its surroundings are inter-related in many ways and can be taken as a group in that region. Hence, option B is correct. Here, the traveler describes the connection to each regional characteristics.

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How did the Byzantine Empire help to shape the modern world?

Answers

Answer: The Byzantine Empire influenced many cultures, primarily due to its role in shaping Christian Orthodoxy.

Explanation:

Answer:

The modern-day Eastern Orthodox Church is the second largest Christian church in the world. In addition, Byzantine art from this period had a strong influence on the later painters of the Italian Renaissance.

Overall, the Byzantine empire has had a wide ranging influence over eastern and even western Mediterranean culture: modern Greece, the Orthodox Church, ancient Greek learning, Muslim and Slavic culture, and more.

A team of marine biologists observe that an inland sea is covered with a green slime. They also observe that besides fishing, the local population
practices large-scale farming in the area. In what possible way can farming be responsible for the state of the lake?
A.
Nutrients from fertilizers on the farm wash off into the lake and cause the green slimy algal bloom
B.
Pesticides from the farm wash off into the lake, causing algae to die and producing a green slime.
C.
D.
E.
Plants growing in the farm increase the chlorophyll levels in the ground, which runs off into the lake, causing the green slime.
The plants in the farm deplete the lake of nutrients and kill off microscopic organisms, causing the green slime.
The green slime in the lake is a toxin produced by pesticides that run off into the lake.
Reset
Next​

Answers

Answer:

A.

Nutrients from fertilizers on the farm wash off into the lake and cause the green slimy algal bloom

Explanation:

This question describes a team of marine biologists, who observe an inland sea covered up with a green slime. Firstly, this green slime is the the visible form of the proliferated growth of algae, hence, causing what is referred to as ALGAL BLOOM.

Algal bloom is caused by EUTROPHICATION, which is the pollution of water bodies with nutrients from fertilizers e.g phosphate, nitrate etc which causes a rapid algae growth. The excessive nutrients causing this algal bloom appearing like a green slime comes from the fertilizers used by farmers practising large scale farming within that area.

When this fertilizer is applied on plants, it gets washed into the water body and its constituents e.g phosphorus, nitrogen etc. serves as source of nutrient for the growth of marine organisms (algae). Hence, they grow excessively and form a bloom that appears like a green slime on the lake.

Hence, the farming activities is releasing pollutants in form of chemical substances which serve as nutrients for the proliferated growth of algae.

Answer:

B.  Pesticides from the farm wash off into the lake, causing algae to die

Explanation:

Green slime algae is a type of algae which is found in the marine ecosystem. This algae is a source of food for aquatic animals. It requires nutrients for producing food in the process of photosynthesis. When the nutrients present in the fertilizers such as nitrates move from the farm into the nearby lakes, its population increases and more food is available for the marine animals and fishing is not possible. But this decrease occurs in population when the pesticides wash off into the lake so farming will be possible for the fisheries.

Hair or fur provide the following- look at picture

Answers

Answer:

Hair and fur provides, insulation, protection, and camouflage

Explanation:

Hair and fur can blend in the environment depending what color it is. For example, a tiger blends into the forest with it's black stripes. They provide insulation as they keep in the heat. They also provide protection from the outside world and from predators as their fur/hair hides them.

The halogens are the most reactive among all

Answers

this is true bruvvvvv

Answer: among all the elements on periodic table

Explanation:

Question 1 (4 points)
True or False: Solar panels are the only way humans can capture energy from the sun
for use.
True
False

Answers

Answer:false

Explanation:because there are a lot of ways that you can get energy.

False will be your answer !

Fiona and her friends were watching a TV show about organisms found in the subtropical forests of southern China. These organisms were acid tolerant and lacked most internal organelles. Which organisms could they most likely be?
A.
amoeba
B.
archaea
C.
dinoflagellates
D.
algae
E.
euglenoids

Answers

I believe the answer is B.Archaea

Answer:

B.   archaea

answer for plato/edmentum

Please answer the following questions about ecology ASAP I always give brainliests and really need help

Answers

Answer:

t

Explanation:

Where is the on and off "switch" located for the neuron?
A. The axon
B. The synaptic gap
C. The dendrites

Answers

Answer:

B.

---------------------

CARBON IS AN IMPORTANT ELEMENT FOR BIOLOGY BECAUSE

Answers

Answer:

D

Explanation:

Carbon is a basis for all life on earth

Ed is analyzing feedback mechanisms that maintain homeostasis in the human body. He follows the procedure that is listed below. (NOTE: Do not conduct this investigation at this time.)

I. Invite a healthy test subject to participate in the investigation.
II. Measure and record the pulse and breathing rate of the subject.
III. Have the test subject exercise vigorously for 1 minute, such as by running in place.
IV. Repeat Step 2.

Why is Step 3 useful for Ed's purpose for the investigation?
a Exercise causes an increase in the pH of blood and other body fluids.
b Exercise increases the body's demand for food and nutrients.
c Exercise has no effect on the body's demand for oxygen, food, and nutrients.
d Exercise increases the body's demand for oxygen.

Answers

Answer:

d. Exercise increases the body's demand for oxygen.

Explanation:

More oxygen is needed during exercise by the body as compared to normal condition because our body needs energy which is only generated in the process of cellular respiration. In cellular respiration, glucose molecule is broken down by mitochondria of the cell and energy is generated in the form of adenine tri phosphate (ATP) which is used for performing muscular activity. So that's why oxygen requirement is more during exercise.


How has population growth led to overpopulation and over-
consumption? Provide two examples from the text to support
your answer.

Answers

Answer:

If we fail to bring population growth and over-consumption under control — the number of people on Earth is expected to grow from 6.5 billion today to 9 billion by the second half of the 21st century — then we will inhabit a planet where life becomes increasingly untenable because of two looming crises: global heating, and the degradation of the natural systems on which we all depend.

Explanation: hope this helps

Sebastian wants to make ball-and-stick models of the four macromolecules. He has colored balls for each of the elements in these molecules, including the following.

red: hydrogen
black: carbon
purple: oxygen
green: nitrogen

Which molecule could he make that consists of long chains of red and black colored balls?

Answers

Answer:

the answer is B lipids :)

hope it helps

Explanation:

Answer:

b

Explanation:

How do the human nervous system and endocrine system interact in
response to a stimulus?
O
A. The endocrine system signals the nervous system to send out
nerve impulses in response to a stimulus.
B. The nervous system signals the endocrine system to release
hormones in response to a stimulus.
C. The nervous system responds to a stimulus, and the endocrine
system returns the body to a relaxed state.
D. The endocrine system responds to a stimulus, and the nervous
system returns the body to a relaxed state.

Answers

Answer: option B.

The nervous system signals the endocrine system to release

hormones in response to a stimulus.

Explanation:

The nervous system respond to stimulus through action potentials and neurotransmitters and they signals the endocrine system to secrete hormones.

The endocrine system is responsible for secreting hormones into the blood which is transported to the target cells. These hormones are responsible for metabolism , growth and response to stress. The hormones secreted provide feedback to the brain so as to affect neural processing.

The leeward side of a mountain receives far less rainfall than the windward side is what?

Answers

Answer: Rain Shadow

Explanation:

Much of airborne moisture falls as rain on the windward side of mountains. This often means that the land on the other side of the mountain (the leeward side) gets far less rain—an effect called a "rain shadow"--which often produces a desert.

Why does sexual reproduction result in more genetic variation in a species than asexual reproduction?

A. In sexual reproduction, offspring have more chromosomes than either parent.

B. In sexual reproduction, offspring are haploid organisms.

C. In sexual reproduction, offspring have fewer genes.

D. In sexual reproduction, offsprings are not identical to either parent.

Answers

Answer°sexual reproduction combines parts of two different genomes to produce offspring,

-Astra

Hope it help pafolloww at pa brain list po salamat

The group of polar bears that live along the eastern coast of Russiam
3
an organism.
a community.
an ecosystem.
a population.

Answers

Answer:

population

Explanation:

quizlet!

Answer:

The answer is D, a population.

Explanation:

Which of the following describes acid rain?
A. coal burning power plants releasing large amounts of sulfur
B. foaming agents or plastics releasing chloroflourocarbons
C. excessive carbon dioxide blanketing the earth
D. overuse of nitrogen-containing materials polluting bodies of water

Answers

Answer:

a

Explanation:

Definition. "Acid rain" is a popular term referring to the deposition of a mixture from wet (rain, snow, sleet, fog, cloudwater, and dew) and dry (acidifying particles and gases) acidic components. ... Unpolluted rain can also contain other chemicals which affect its pH (acidity level).

A.Coal burning power plants releasing large amounts of sulfur

If you exert 200 N of force to move a box 8 meters across the living room in 2

seconds, what is your power?

A. 80,000 W

OB. 400 W

C. 1600 W

O D. 800 W

SUBMIT

Answers

Answer:

800 W

Explanation:

Power is the rate at which work is done. That is, time taken to do a certain amount of work.

Mathematically, power = work done ÷ time taken

But work done = force × distance/displacement

Then work done = 200 N x 8 m = 1600 Nm = 1600 J (since 1 Nm = 1 J)

So power = 1600 J ÷ 2 s = 800 Js^(-1) = 800 W (since 1 Js^(-1) = 1 W).

1. Calculate If 1000 units of energy are available at
the producer level of the energy pyramid, about
how many units are available for first-level con-
sumers? Second-level consumers? Third-level
consumers?

Answers

Answer:

first-level consumers- about 100units

Second-level consumers- about 10units

Third-level consumers- about 1unit

Explanation:

A food chain represents the series of energy transfer from one organism to another. This energy is transferred when one organism feeds on another organism. A food chain always starts with a PRODUCER, which is an organism capable of producing its own food via photosynthesis. The producer creates the energy that is transferred as the food chain progresses.

However, only about 10% of the available energy in one organism is transferred to another, this is because most of the energy (about 90%) is lost as heat when the organism undergoes metabolic activities. Hence, if 1000units of energy are available at the producer level, a primary/first level consumer will feed on the producer and 10/100 × 1000 = 100units of energy will be available for the primary/first level consumer.

Likewise, 10% of 100units= 10 units of energy will be available for the second level consumers when it feed on the first level consumers. Lastly, 10% of 10units = 1 unit will be available for third level consumer when it feeds on second level consumer.

Bacteria will not be found on fruits or vegetables.

True
False

Answers

Answer:

It's false, bacteria will be found

Explanation:

Answer:

i think its false because some of them have pores which allow bacteria to get in

Explanation:

Maritime polar air masses _____.

A. bring clear, cold, dry air to much of North America

B .are warm, humid air masses that form over oceans

C. bring hot, dry weather to the southern Great Plains

D .are cold, humid air masses that bring fog to the West Coast

Answers

Answer:

d.

Explanation:

How does the change in estrogen (estradiol) levels correspond to the uterine (endometrial) thickness on days 5-14?

Answers

Why is understanding mitts important?( Select the best answer)

1.It helps us understand how organisms repair.

2. It helps us understand how organisms grow.

3. It is important for cancer research.

4. All options correct.

Which symbol is used to label a male that expresses a sex-linked disorder on a pedigree? An empty circle. A half-filled circle. An empty square. A filled square.

Answers

A Filled square is used to label a male that expresses a sex-linked disorder on a pedigree. So, the correct option is D.

What is Pedigree chart?

A pedigree chart is defined as the expression of phenotypic traits in the progenitor and the descendants of different generations in which the circle represents a female and the square represents a male.

If the whole figure is shaded or colored it means that the person is affected. In the parental generation, a single female mates with two males to produce the boy in the first generation, while the sex of the offspring is always dependent on the sperm cell containing the Y chromosome.

When an egg cell with a single X chromosome is fertilized with a sperm containing a Y chromosome it results in a male or if it binds to a sperm having an X chromosome it results in a female. Generally, there is a genetic cross between a male and a female. 50% result in either sex in the offspring.

Thus, a Filled square is used to label a male that expresses a sex-linked disorder on a pedigree. So, the correct option is D.

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A food chain is illustrated below:
The arrows represented most likely indicate

A. Energy released from metabolism into the environment as heat
B. Oxygen released from metabolism produced by cellular respiration
C. The absorption of energy from the environment that has been created
D. The transport of glucose away from the organism through photosynthesis

Answers

Answer:

A. Energy released from metabolism into the environment as heat.

Explanation:

Hope it helped pls mark me brainliest

The arrows represented most likely indicate is Energy released from metabolism into the environment as heat.

What is the food chain?

A food chain describes how energy and nutrients move through an ecosystem. At the basic level there are plants that produce the energy, then it moves up to higher-level organisms like herbivores. After that when carnivores eat the herbivores, energy is transferred from one to the other

Thus, option 'A' is correct.

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What do the variables in the Hardy-Weinberg equation represent?

p
p2
q
q2
2pq

Answers

Answer:

p represents the frequency of the dominant allele (in decimal form) of a gene

q represents the frequency of the recessive allele (in decimal form) of a gene

These 2 alleles are different; one is dominant, one is recessive.

p^2 = the frequency of homozygous dominant individuals in the population (decimal form)

2pq = the frequency of heterozygous individuals in the population (decimal form)

q^2 = the frequency of homozygous recessive in the population (decimal form)

How are tumor cells different from normal cells?

A. There are chromosomes in tumor cells.

B. Mitosis is not regulated in tumor tumor cells.

C. Tumor cells use meiosis to replicate.

D. Tumor cells are haploid and other cells are diploid.

Answers

Answer:

B

Explanation:

Where does the energy come from to make NADPH in the light reactions?
A. From the energy released by ATP molecules
B. From the energy stored in light
c. From the energy stored by the Calvin cycle
D. From the potential energy of ADP molecules
SUBMIT

Answers

Explanation:

Figure 19.2. The Light Reactions of Photosynthesis. Light is absorbed and the energy is used to drive electrons from water to generate NADPH and to drive protons across a membrane. These protons return through ATP synthase to make ATP.

Answer:

Explanation:

B. From the energy stored in light

Other Questions
Answer correctly !!!!!! Will mark brainliest !!!!!!!! How do you solve an standard form/ intercept in y=4x-10 ? Help me plzzzz :/ Oscar owns a bulldog. Another dog owner filed a lawsuit against Oscar alleging that his bulldog injured her pet poodle in a dog park fight. Despite evidence that his bulldog was not present at the dog park when a dogfight broke out, the jury found Oscar liable for injuries caused to the poodle. Based on these facts, Oscar has the legal option to do which of the following?A. Make a peremptory challenge (has to do with a juror).B. File a motion or a judgement notwithstanding the verdict.C. Motion that the court nullify the verdict based on res judicata (no this has to do with not refiling the case. ASAP WILL DO BRAINLIESET Karen can complete 12 coloring book pages in 30 minutes.a) what is the constant of proportionality? b) Write a direct variation equation that represents the scenario c) How long will it take Karen to complete 96 coloring pages? How do you write 345,000,000,000 in scientific notation A grocery store clerk can scan one product every 1/2 of a second at what rate Can she scan Products Which angle pairs are supplementary? Check all thatapply.21 and 220 24 and 230 24 and 25O 27 and 25O 23 and 26 What details from a story best help develop the theme?-belreaders' prior knowledgethe author's motivations for writingdescriptions of time and placecharacter motivation and plot what is the total amount of sap produced that day? What happens in the first part of Interphase? In the essay "Nonviolence and Racial Justice" (1957), Martin Luther King, Jr., asks, "How is the struggle against the forces of injustice to be waged?" Answer his question by arguing which strategies best helped civil rights activists and activists from current equal rights/justice movements achieve the goal of overcoming injustice. Draw on specific evidence from the civil rights movement to support your answer. Write an essay analyzing the issue raised by the above question and arguing for a specific position. Through a well-developed thesis statement, support your argument by providing textual evidence and examples from the provided articles and sources. Please be sure to cite every piece of evidence you use from the articles. Your essay should be clearly focused, well organized, and carefully written. The Boston Massacre is an example of Please answer this correctly Which quantum number describes the energy level of an electron?Spin quantum numberPrincipal quantum numberMagnetic quantum numberAngular momentum quantum number 37 is 60% of what number? y varies inversely as x, if y = -3 when x = -6, find y when x = 14. A line segment 9 cm long is dilated with a scale factor of 3. Find the length of the dilated line segment. A. 1/3 cm B. 3 cm C. 12 cm D. 27 cm Solve the equationThe equation is -3 = 8x -4y for y An investor is considering buying a restaurant that has been in operation for a number of years. The restaurant has a highly regarded chef and many long-term kitchen and wait staff who work together smoothly to make innovative new dishes. It has a reputation for dishes of consistently high quality and an appealing dining atmosphere. What should the investor consider when making a decision? Solve for x. 2(4x - 1) + 3(3 - 2x) = 0