Which is the role of restriction enzymes?
to isolate the selected gene
to cut DNA into fragments of different lengths
to move and separate the strands of DNA
to join the sticky ends of DNA fragments

Answers

Answer 1

Answer:

to cut DNA into fragments of different lengths

Explanation:

A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence.

More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. In live bacteria, restriction enzymes function to defend the cell against invading viral bacteriophages. Restrictions sites in the viral genome (a "happy accident" of nature, as far as the bacteria are concerned, since they don't appear to have any specific function in the virus) are cleaved by the bacterium's restriction enzymes, fragmenting and destroying the DNA of invading bacteriophages before it can incorporate into the host's genome and take over the cell.

A bacterium is immune to its own restriction enzymes, even if it has the target sequences ordinarily targeted by them. This is because the bacterial restriction sites are highly methylated, making them unrecognizable to the restriction enzyme.

Isn't evolution fantastic?

Answer 2

The correct answer is option C i.e. to cut DNA into fragments of different lengths.

what is  restriction enzyme?

A restriction enzyme is a bacterial protein that cleaves DNA sequences at sequence-specific places, resulting in DNA fragments with known sequences on both ends.

Certain laboratory processes, such as recombinant DNA technology and genetic engineering, require the use of restriction enzymes.

There are four different types of restriction enzymes: type I, II, III, and IV, which differ principally in structure, cleavage site, specificity, and cofactors.

Both restriction and methylase functions are carried out by a single big enzyme complex in Types I and III enzymes.

The restriction enzyme is independent of its methylase in the Type II system.

Several thousand type II restriction enzymes have been identified from a variety of bacterial species.

These enzymes recognize a few hundred distinct sequences, generally four to eight bases in length.

Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites.

Type IV restriction enzymes cleave only methylated DNA and show weak sequence specificity.

hence, the correct answer is option B.

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Related Questions

Volcanoes are usually formed as a result of _____.

a transform fault boundary, where both plates are oceanic
a convergent boundary, where both plates are continental
a convergent boundary, where at least one of the plates is oceanic
a divergent boundary, where at least one of the plates is oceanic

Answers

Answer:

a transform fault boundary, where both plates are oceanic

Explanation:

A volcano is formed when hot molten rock, ash and gases escape from an opening in the Earth's surface. The molten rock and ash solidify as they cool, forming the distinctive volcano shape shown here. As a volcano erupts, it spills lava that flows downslope.

Answer:

a transform fault boundary, where both plates are oceanic

Explanation:

What is produced when an atom loses an electron?

Answers

Answer:

It becomes a positively charged ion.

Explanation:

Answer:

it will lose negative charge and become positively charged

could someone please help me out on this? im confused​

Answers

Answer:

Dependent: Reduction

Independent: Solar Energy

Controlled: B

Explanation:

The dependent variable is what is being measured in the lab.

The independent variable is what is being CHANGED by the individual

The control is what is kept the SAME throughout the lab.

Hope this helped!

Many people try to eliminate fat from their diets. Which is one reason it is
necessary for humans to eat fat?

Answers

Answer:

the internal organs of the body is cushioned by fat. fat is adipose tissue stored in the body. fat is as important as such other micro molecules such as protein carbohydrates. they serve the body in many ways out of those one of the important function is protection of internal organs.

What should Jill’s conclusion be?

Answers

Answer:

the conclusion should be... depending on the amount of fertilizer you use it will have more or fewer flowers on each plant due to a lot of fertilizer or less. hope this helps have a good day.

can someone please help me ..

Answers

Is there a word bank?

Which of the following best describes the relationship between cells and living things in general?

Answers

You need to add the pictures for us to see..

How was the process used to construct the tree of life by Carl Woese different from the process used by Jill Banfield?

Answers

Answer and Explanation:

Carl Woese used ribosomal RNA genes to study the evolution of living beings. He used these genes because they are present in all organisms, and they all have the same function. The nucleotide sequences change very little, giving a real evolutive vision. Woese compared ribosomal genes among different living beings. The sharper the sequence variation is among two organisms, the bigger the evolutive divergence is. This divergence drowned in a phylogenetic tree shows three well-differentiated domains: Archea, Bacteria, and Eukarya. Jillian Banfield used genes from 16 ribosomal proteins from many different unknown microorganism species. She and her team used metagenomic methods that are the simultaneous analysis of multiple genomes belonging to many organisms in the same sample. These methods allowed detecting different organisms, even when these were neither previously observed nor lab-cultivated. The comparison among these new genomes with many others already known made a place for the construction of a new and more detailed tree of life where many new branches emerged from the three domains.

5 points
Transmembrane proteins span the width of the cell membranes. Four types of transmembrane proteins are shown in a section of cell membrane.
groene
Although these proteins have different specific functions, they all-
A-stop chemical reactions within the cell

B-synthesize molecules that signal other cells

C- help the cell interact with its external environment

D- remove large waste particles from the cytoplasm of the cell

Answers

Answer:

C. help the cell interact with its external environment

Explanation:

A transmembrane protein (TP) is a type of integral membrane protein that spans the entirety of the cell membrane. Many transmembrane proteins function as gateways to permit the transport of specific substances across the membrane.

Although transmembrane proteins have different specific functions, they always HELP the cell interact with its external environment.

Transmembrane proteins are a broad category of membrane proteins that act as carriers and/or transporters in order to allow the movement of specific substances across cell membranes.

After binding to their specific ligands, the three-dimensional (3D) shape of transmembrane proteins changes in order to allow the movement of molecules and ions through the cell membrane.

For example, aquaporins are a type of transmembrane protein that regulates the entry of water (H2O) molecules into and out of cells.

In conclusion, although transmembrane proteins have different specific functions, they always HELP the cell interact with its external environment.

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list 5 types of bacteria d their unique home.​

Answers

Five types of bacteria: coccus, bacillus, spirillum, rickettsia, and mycoplasma, their classified to their basic shapes.

Pls help I need the answers ASAP


Balance the following equations. A few of the equations are balanced and will not need
anything done to them. If the equation is already balanced write the word balanced as
your answer.

Answers

Answer:

1) 6K + B2O3 ---> 3K2O + 2B

2) 4C + [tex]S_{8} \\[/tex] ---> [tex]4CS_{2}[/tex]

3) 2Na + O2 ---> [tex]Na_{2}O_{2}[/tex]

4) 2N2 + SO2 ---> [tex]2N_{2}O_{5}[/tex]

5) Balanced

6) 4Na + O2 ---> [tex]2Na_{2} O[/tex]

7) 16Al + [tex]3S_{8}[/tex] ---> [tex]8Al_{2}S_{3}[/tex]

8) Balanced

9) Balanced

Explanation:

Which of the following is NOT a photosynthetic pigment?

Cartenoids
cytokines
Chlorophyll
Xanthins

Answers

B because that’s apart of the cell cycle i’m pretty sure

Choose all that apply. Carbohydrates contain the following elements:

Answers

Answer: carbon, hydrogen, and oxygen

Explanation:

hydrated carbon which results to sugar and oxygen

Explanation:

plz give me brainiest

Which best explains how to oxygen Atoms,
each with six valence electrons can bond with each other?

Answers

Explanation:

Two oxygen atoms bonds with each other via covalent bonding.

In the formation of covalent bonds, two atoms with little to no electronegativity difference combines by sharing of the valence electrons. This why homonuclear molecules can be formed.

Two atoms of the same kind having zero electronegativity difference forms this covalent bonds. There is equal sharing of electron pair between the atoms and there is no electrostatic charge on the molecule.

For the oxygen atom with 6 valence electrons;

   two electrons are shared by each atoms to make a complete octet and a stable molecule.

I give a brainliest who answers this question. What kind of chemical reaction does this energy diagram show?

look the Photo.


A) exothermic

B) endothermic

C) ionic

D) mass creating

Answers

Answer:

b) endothermic

Explanation:

please help me on this

Answers

Answer:

secondary succesion

Explanation:

Answer:

its

primary succession

i hope the answer was helpful

Although Arroyo does not mention the term "watershed," it was discussed in the unit as being closely related to and impacted by drought and floods. Please discuss how the watershed relates to drought and heat. How might Arroyo have integrated the term watershed into her talk?

Answers

Answer:

The land between the water has become more prevalent because due to droughts, the water has evaporated and dropped.

Explanation:


4. Why must the process of meiosis create only half the number of
chromosomes (23) in each new cell? *
A. The cells are smaller.
B. Egg and sperm cells must only have the number of chromosomes so that when
they combine the number will equal 46.
C. 72 the number of chromosomes are not produced in this process, 46, the total
number is produced.
D. If not, the cell would die.

Answers

Answer:

I think it's B.

Explanation:

hope this helps

Answer:

B. Egg and sperm cells must only have the number of chromosomes so that when they combine the number will equal 46.

Explanation:

The purpose of meiosis is to shuffle genetic information and cut the cellular chromosome number in half, from 46 chromosomes to 23 chromosomes. In this way, when an egg and sperm cell combine during fertilization, the resulting embryo will inherit the appropriate amount of unique genetic information from each parent.

Hope this helps!!

A basketball with a mass of 0.60 kg is accelerated with a force of 10.8 N. If resisting forces are ignored, what is the acceleration of the basketball to the nearest m/s2?

Answers

Answer:

18 m/s^2 A newton is defined as kg*m/s^2 and you want to accelerate a mass in kg, getting a result of m/s^2. So what you need to do is cancel out the kg unit in the newtons. You can do that by dividing the newtons by kg. So let's do the math. 10.8 kg*m/s^2 / 0.60 kg = 18 m/s^2 So the ball has an acceleration of 18 m/s^2. Remember, look at the units you have and the units you desire. By doing so, the math operations become obvious.

Explanation:

Answer:

18 m/s^2

Explanation:

18 m/s^2 A newton is defined as kg*m/s^2 and you want to accelerate a mass in kg, getting a result of m/s^2. So what you need to do is cancel out the kg unit in the newtons. You can do that by dividing the newtons by kg. So let's do the math. 10.8 kg*m/s^2 / 0.60 kg = 18 m/s^2 So the ball has an acceleration of 18 m/s^2. Remember, look at the units you have and the units you desire. By doing so, the math operations become obvious.

Which gas is released during the light reactions of photosynthesis?

Answers

Would say carbon dioxide

The diffusion of water into or out of a cell is called

solubility
solubility

osmosis
osmosis

selective transport
selective transport

endocytosis

Answers

It’s called osmosis.

Oxygen enters a cell via:
Active transport
Diffusion
Filtration
Osmosis

Answers

Diffusion

Hope this helps

Answer:

Oxygen and carbon dioxide move across cell membranes via simple diffusion, a process that requires no energy input and is driven by differences in concentration on either side of the cell membrane.

Explanation:

Simple diffusion is responsible for the spread of an air freshener through a room, or of a few drops of dye to eventually turn a glass of water a uniform color. Molecules naturally disperse from areas of higher concentration to lower concentration.

As oxygen-rich (and carbon dioxide-poor) blood travels by a cell the oxygen diffuses through the cell membrane to the area of lower concentration inside the cell. It can do this easily because the oxygen molecule (O2) is very small and has no charge or polarity. The oxygen is used up rapidly by mitochondria. This rapid consumption causes oxygen to constantly move into the cell from the blood.

The mitochondria creates carbon dioxide (CO2) as a waste product of cellular respiration (the process that makes energy for your body). Because the CO2 is of a higher concentration in the cell than in the blood passing by, this gas continually diffuses out of the cell. It too is small and uncharged so it can pass through cell membranes easily.

These movements require no energy (in the form of ATP) on behalf of the cell.

how does food go bad in what ways can food be preserved​

Answers

Due to microbial and bacterial attack the food get spoiled.

We can preserved the food by:

Sun dyingcooling in refrigeratorsaltingchemical preservatives like, sodium benzoate

PLEASE GIVE ME AS BRAINLIEST


What would be the main problem with reproduction if meiosis did not take place to form sex cells?

Answers

Answer:

Fertilization would result in a zygote with twice as many chromosomes as the parents.

Explanation:

Meiosis creates four haploid cells from a diploid parent cell. These haploid cells are the sex cells - sperm cells and egg cells.

Since these cells are haploid (containing 23 chromosomes, half the amount of chromosomes as the parent diploid cells), when they fuse during fertilization, they form a diploid zygote containing 46 chromosomes.

Answer:

B) Fertilization would result in a zygote with half as many chromosomes as the parents.

Explanation:

Of the following statements, WILL MARK YOU BRAINLIEST!!

which is not part of the current rules of animal research?
A. The research must have a clear, scientific purpose.
B. The research must provide humane care and housing.
C. The research must ensure the least amount of suffering possible.
D. The research must use animals whenever possible.

Answers

Answer:

Answer D

Explanation:

Because for animal research, you want to use animals as little as possible

Answer:

D: The research must use animals whenever possible.

Explanation:

I got it right on edge

Name two processes that are important in the carbon cycle.
Options: a. Photosynthesis and nitrification b. Nitrification and respiration
c. Photosynthesis and respiration d. Nitrification and denitrification

I'm thinking it's A but am unsure.

Answers

Answer:

I think it's C, sorry if I'm wrong. I just took this class.

What is genetic tagging?
O a technique that uses specific antibodies to
visualize features of cells
O a technique that visualizes how specific
genes are used within a cell
O a technique in which cells are purposefully
grown under specific conditions
o an imaging technology used to study features
smaller than the human eye can see

Answers

Answer:

a technique that visualizes how specific

genes are used within a cell

Explanation:

31.
The chemical equation below represents a chemical process.
C6H12O6 + 602 6H2O + 6CO2 + ATP
What chemical process is represented by the equation?
A. This process occurs in anaerobic respiration.
B. The process is oxidation in which plants obtain energy.
C. The process is photosynthesis in which plants obtain food and energy in the form of ATP.
D. The process is cellular respiration which chemical energy from food sources is released in
the form of ATP.

Answers

Answer:

D

Explanation: D. The process is cellular respiration which chemical energy from food sources is released in

the form of ATP.

Which cells are most likely to increase in number as a result of this adaptation?
neurons
red blood cells
white blood cells
muscle cells

Answers

Answer: red blood cells

Explanation: took the test haha

Suppose a scientist must choose whether to publish a report in a newspaper or in a peer-reviewed journal. What is a benefit of publishing in the journal?

Answers

Answer/Explanation:

Because the journal is peer-reviewed, it means their report is assessed and critiqued by experts in the field. This means the data is more reliable and  more likely to be trusted by other scientists in the field.

Additionally, the journal is likely to be specialized and used to accepting scientific reports, meaning they will have certain regulations and criteria for publishing.

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The table shows four account transactions. Find the account balance after the transactions when the account started with a balance of $55. Why was there great concern about admitting Missouri as a slave state? It meant that Maine could not have slaves. The Louisiana Purchase had required that there be no slave states within the territory. Transporting slaves to Missouri was difficult. There would be more slave states than free states. Help!!!!!!!!! Quick!!!!! i need 2 quotes from the first hunger games book supporting that haymitch is an antagonist/bad guy. they have to be from the first book btw!! Flaws occur at random along the length of a thin copper wire. The number of flaws per mm follows a Poisson Distribution with a mean of 2.3 flaws per mm. Determine the probability of exactly 2 flaws in 1 mm of wire. Can someone help me PLEASE?!!! will mark brainliest and also the rest!!!can someone write them in their OWN words?!!! PLEASE!!! (this is for Science)THANK YOU SOOO MUCH FOR WHO EVER HELPS ME!!! REALLY APRICIATE! THX :) HELP PSYCOLOGY Read the following scenario and answer the question that follows.Marley and Soncy witnessed their classmate, Josh, get into trouble by their fourth grade teacher. Marley claimed thatJosh's behavior was wrong because he hurt another student and Soncy claimed that Josh's behavior was wrongbecause he broke the class rules.Based on Lawrence Kohlberg's theory of moral reasoning which of the following statements would Kohlberg agree with?A Marley is at a higher level of moral reasoning than Soncy.B. Soncy is at a higher level of moral reasoning than MarleyC. Soncy and Marley are at the same level of moral reasoningD. Marley reason's differently than Soncy because Marley is more mature Rebecca Smith was the top scorer in a women's professional basketball league for the 2006 regular season, with a total of 863 points. 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